Separation of small ciliate protozoa from bacteria by sucrose gradient centrifugation

Separation of small ciliate protozoa from bacteria by sucrose gradient centrifugation.

Small ciliate protozoa of the genus Uronema were separated from labeled bacteria by means of a discontinuous sucrose gradient. More than 98% of the radioactivity associated with the bacteria was found in the upper portion of the gradient, whereas the remaining portion of the gradient contained the ciliates.

Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification...

Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans

Separation of Hela Cells by Colloidal Silica Density Gradient Centrifugation

Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040-1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were ar...

Rumen holotrich ciliate protozoa.

Factors Influencing Size of the Protozoal Population .................................................................. 28 Diurnal Variations in the Holotrich Population ............................................ ......................28 IN VITRO CULTIVATION OF HOLOTRICH PROTOZOA...........................................................28 TECHNIQUES FOR ISOLATION OF HOLOTRICH CILIATES FROM R...